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airSEM™, High resolution SEM operating in open air a new approach for correlative imaging of hydrated samples

Abstract:

The airSEM™ represents a new approach to electron microscopy, allowing to image objects close to their natural state and simplifying the integration of multiple imaging techniques on a single imaging platform.

In order to demonstrate these advantages, two representative case studies are presented:

  1. Correlative fluorescence SEM imaging of lung extracellular matrix: Collagen type I is specifically localized by direct correlative imaging of immunofluorescence and SEM tissue morphology.
  2. Calcium uptake in sea urchin embryos: Fluorescently labelled mineral is specifically localized, in fully hydrated minimally processed sea urchin embryo section, by correlation to the SEM image.

 

Concept of imaging station:

The airSEM™ imaging station includes multiple imaging modalities on a single platform, sharing a common sample stage. The platform is tailored to image the same region of interest with various imaging modalities.

The relative locations of the different imaging axes on the platform are fixed and pre‑calibrated; one can easily and accurately alternate between the different modalities. These characteristics make the airSEM™ imaging station an ideal solution for correlative microscopy.

airSEM Imaging Station

 

airSEM Vs Vacuum SEM

Link to Poster:

airSEM a new approach for correlative imaging of hydrated samples – IMC 2014