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Introduction of correlative light and airSEM microscopy imaging for tissue research under ambient conditions

Abstract:

A complete fingerprint of a tissue sample requires a detailed description of its cellular and extracellularA�components while minimizing artifacts. We introduce the application of a novel scanning electronA�microscope (airSEMa�?) in conjunction with light microscopy for functional analysis of tissue preparationsA�at nanometric resolution (<10 nm) and under ambient conditions.

Our metal-staining protocols enableA�easy and detailed visualization of tissues and their extracellular scaffolds. A multimodality imaging setup,A�featuringA�airSEMa�?A�and a light microscope on the same platform, provides a convenient and easy-to-useA�system for obtaining structural and functional correlative data.

TheA�airSEMa�?A�imaging stationA�complementsA�other existing imaging solutions and shows great potential for studies of complex biological systems.

Corelative Imaging3

Link to paper:

Introduction of correlative light and airSEM microscopy imaging for tissue research under ambient conditions

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