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Introduction of correlative light and airSEM microscopy imaging for tissue research under ambient conditions

 

Affiliation:

This work was done In collaboration with Inna Solomonov, Dalit Talmi-Frank, Anna Aloshin & Irit Sagi Department of Structural Biology

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Abstract:

A complete fingerprint of a tissue sample requires a detailed description of its cellular and extracellular components while minimizing artifacts. We introduce the application of a novel scanning electron microscope (airSEM™) in conjunction with light microscopy for functional analysis of tissue preparations at nanometric resolution (<10 nm) and under ambient conditions. Our metal-staining protocols enable easy and detailed visualization of tissues and their extracellular scaffolds. A multimodality imaging setup, featuring airSEM™ and a light microscope on the same platform, provides a convenient and easy-to-use system for obtaining structural and functional correlative data.  The airSEM™ imaging station complements other existing imaging solutions and shows great potential for studies of complex biological systems.

 

Link to PDF

Solomonov, I. et al.

 

Link to paper:

http://www.nature.com/srep/2014/140807/srep05987/full/srep05987.html