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Introduction of correlative light and airSEM microscopy imaging for tissue research under ambient conditions

 

Affiliation:

This work was done In collaboration withA�Inna Solomonov, Dalit Talmi-Frank, Anna Aloshin & Irit SagiA�Department of Structural Biology

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Abstract:

A complete fingerprint of a tissue sample requires a detailed description of its cellular and extracellularA�components while minimizing artifacts. We introduce the application of a novel scanning electronA�microscope (airSEMa�?) in conjunction with light microscopy for functional analysis of tissue preparationsA�at nanometric resolution (<10 nm) and under ambient conditions. Our metal-staining protocols enableA�easy and detailed visualization of tissues and their extracellular scaffolds. A multimodality imaging setup,A�featuring airSEMa�?A�and a light microscope on the same platform, provides a convenient and easy-to-useA�system for obtaining structural and functional correlative data. A�The airSEMa�?A�imaging station complementsA�other existing imaging solutions and shows great potential for studies of complex biological systems.

 

Link to PDF

Solomonov, I. et al.

 

Link to paper:

http://www.nature.com/srep/2014/140807/srep05987/full/srep05987.html

 

 

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