Sample Preparation

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Sample Preparation

airSEM™ offers a paradigm shift with regards to sample preparation for electron imaging.

Beyond sidestepping almost all sample preparation, in itself rendering the airSEM™ a highly convenient electron microscope, the two major advantages offered are:

Solid state samples can be directly imaged without coating, which, in many cases, can obscure critical information.

Life Sciences: Current preparation procedures for vacuum SEM have several known drawbacks, all of which are eliminated when using the airSEM��� platform:

  1. Artifacts:  Standard preparation procedures can alter fine morphological details.
  2. Information loss: Stripping of water-soluble chemical groups, such as fluorescence markers.
  3. Destructive sample preparation: Blockage of the possibility to image the sample again with fluorescence microscopy.
  4. Time consuming: some of the widespread sample preparation techniques can take days to implement properly.

A comparison of sample preparation steps for tissue slices for both vacuum SEM and airSEM™ is presented:

Sample Preparation table

 

Sample Preparation_3

airSEM��� surpasses the steps of fixation, dehydration, drying or coating which in many cases alter the chemistry of the sample and its structure and may introduce artifacts